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Next, we carried out experiments in vitro to further corroborate the role of USP2 in the progression of TGF1-induced ferroptosis

The release medium was phosphate-buffered saline, prepared according to pharmacopeial standards and consisting of 0.02% KCl (w/v), 0.02% KH 2 PO 4 (w/v), 0.116% Na 2 HPO 4 (w/v), and 0.8% NaCl (w/v) (adjusted with 10 mM NaOH, pH 7.4) ( The CUR was formulated in a 2% polysorbate 80 aqueous solution, achieving a final concentration of 0.6 mg/mL

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