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Primary anti- RIP3(ab62344, Abcam, Cambridge, MA, USA), anti-MLKL (ab196436, Abcam, Cambridge, MA, USA), anti-TNF- (ab183218, Abcam, Cambridge, MA, USA), anti-IL-6 (ab229381, Abcam, Cambridge, MA, USA), anti-ZO-1 (Abcam, USA), anti-Occludin (Abcam, USA), and anti-GAPDH (CST) antibody were then applied, and anti-GAPDH antibody was employed as the loading control

10.1016/j.pain.2006.01.037 78 ForsythP

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This hormonal profile suggests DSIP may support anabolic processes, tissue repair, and metabolic health beyond its sleep effects