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Evrensel A, Ceylan ME

Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

All participants were aged between 50 and 76 were recruited in the state of Washington between the years 2000 and 2002

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One of the many side effects of these steroids is male infertility and testicular shrinkage