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Trace metal ions like iron and copper catalyzing oxidation through redox cycling, light-accelerated degradation, ambient oxygen in your environment all these things convert your GSH to GSSG faster than most people realize

Therefore, searching for potential adjuvant therapies with hepatoprotective capacities to be used during chemotherapy is widely adopted (Liang et al., 2021) with the need to understand the mechanisms underlying MTX-induced liver toxicity

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Appl Microbiol Biotechnol 66:233242 Li W, Li Z, Ye Q (2010) Enzymatic synthesis of glutathione using yeast cells in two-stage reaction