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Since then, the city changed the way it chose contractors for large projects, allowing for the best value as opposed to the lowest responsible bidder. This change, Hess said, allowed the city to go for contractors that fit the city and projects specific needs

A large fragment (LgBiT) of the NanoBiT luciferase was inserted into the helical domain (between the A and the B helices) of a G subunit with 15-amino acid flexible linkers

for others, it becomes a barrier to long-term consistency

Code of Federal Regulations (eCFR)

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(5) The apparent closure rate was three times faster than standard care