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The enzyme dipeptidyl peptidase-4 (DPP-4) cleaves native GHRH within minutes by removing the first two amino acids from the N-terminus, rendering it biologically inactive

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doi: 10.1210/jc.2006-0099 35 SawinCT

Sample analysis was performed on an Agilent 1290 UPLC system equipped with a Waters BEH amide (2.1 mm 100 mm, 1.7 m) column using a gradient of 15 mM NHAc/0.3% NHOH in HO (A) and 90% ACN (B) at 0.3 mL/min

This distinction is important because endothelial behavior is highly sensitive to experimental context

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