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doi: 10.3390/life14050606 [DOI] [PMC free article] [PubMed] [Google Scholar] 112.Huang F, Guo WJ

doi: 10.1007/s12016-021-08903-z [PubMed] 36036421 Rukasin CRF, Phillips EJ, Stone CA Jr

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additionally, anyone experiencing breathing or swallowing issues should seek urgent care as these may indicate distant spread of the toxin

2001;532(Pt 2):42334

The cell pellet was washed once with 1x PBS supplemented with 100 g/mL cycloheximide before resuspending it in Lysis Buffer (20 mM HEPES/KOH pH 7.5, 150 mM KOAc, 5 mM MgCl 2 , 1 mM DTT, 0.5% IGEPAL CA-630 (Sigma-Aldrich), protease-inhibitor mix (homemade), 100 g/mL cycloheximide, 2 U/mL SUPERaseIn RNase Inhibitor (Thermo Fisher Scientific))