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Human clinical trials are lacking

In recent years, increasing numbers of researchers have delved into this hotspot and found that the mechanisms included: secretion of growth factors such as BDNF [36, 37], neuroplasticity enhancement [38], improved brain oxygenation and nutrient supply [39], and reduced inflammation to protect neurons [40]

Permeability changes have not been verified yet in the constipation-predominant IBS (IBS-C) subgroup by other investigations, whereas patients with mixed bowel habits (IBS-M) have not been investigated so far

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Exosomes (30150 nm) are a subtype of EVs that originate from the endosomal pathway in cells and are secreted into biological fluids, including plasma, urine, saliva, and cerebrospinal fluid (Ebadpour et al., 2025

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