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If the pH is above 10, ammonia gas may be released from the drilling fluid

Additionally, for follow-up studies, it would be interesting to include a control with heat-inactivated HIV in the MNO condition, to be able to better differentiate between replication-dependent and independent effects of the virus in the presence of microglia

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Based on mass, HDL can also be separated from other lipoproteins by size-exclusion chromatography (fast protein liquid chromatography, FPLC), and HDLs molecular weight ranges from 175,000 - 360,000 Da (762)

Rowland AS, Baird DD, Weinberg CR, Shore DL, Shy CM, Wilcox AJ

The supernatant was aspirated and the cell pellet was suspended in supplemented DMEM media for further experiments