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84,85 Briefly, synchronized L1 animals were seeded onto NGM plates and grown until the L4 stage

In order to maximize the solubility of proteins, a calculated volume of Waters Rapigest (Waters Corporation, Milford, MA, USA) was added to yield final concentrations of 0.10.2% Rapigest in pre-digestion samples

Short ncRNAs consist of microRNAs (miRNAs), small interfering RNAs (siRNAs) and Piwi-associated RNAs (piRNAs)

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