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Researchers are advised to monitor the subjects total copper levels and incorporate zinc supplementation if necessary

Same fucking song, not wanting to be there anymore
The apical supernatant was used for GSH:GSSG and protein analysis

Dujardin, S

Regulation of hepatic lipogenesis by the transcription factor XBP1

Following 30 min of incubation at 4 C, extracts were cleared by centrifugation (17,000 g , 20 min, 4 C) and then supernatants were analysed by liquid chromatography and mass spectrometry (LCMS)
