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After the water bath digestion, the samples were allowed to cool at room temperature and then 2mL of HClO 4 were added, and thereafter was heated on hotplate at 160 0 C for 5 minutes until the white dense fumes of HClO 4 were released

[DOI] [PubMed] [Google Scholar] [211].Ungard RG, Seidlitz EP, Singh G, Inhibition of breast cancer-cell glutamate release with sulfasalazine limits cancer-induced bone pain, Pain 155(1) (2014) 2836