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AIF is a protein located within the mitochondria, whose activation leads to a caspase-independent pathway of apoptosis by mediating DNA fragmentation and chromatin condensation [49]

Clinical studies have demonstrated the feasibility of both approaches, with oral DCA being well-tolerated in patients with recurrent malignant brain tumors and Lonidamine showing modest efficacy in a Phase II study for recurrent glioma, thereby providing a clinical foundation for targeting glycolytic flux to suppress tumorigenic lactate generation [224, 225]

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