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Papain (50 g/reaction) was activated in the assay buffer (50 mM Tris-HCl pH 7.6, containing 2 mM cysteine and 0.1 mM EDTA, pH 6.5) for 30 minutes at 40C, and the papain substrate was diluted in DMSO

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N Engl J Med), in 99 individuals who had undergone various solid organ transplant procedures (heart, kidney, liver, lung, pancreas) 978 months previously who received a third vaccine dose, the adverse event profile was similar to that after the second dose and no grade 3 or grade 4 events were reported in recipients who were followed for 1 month following post Dose 3

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