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No peer-reviewed human trial data exists

Recordings were made at room temperature in artificial cerebrospinal fluid (aCSF) (pH 7.3, osmolarity 305 +/- 5 mOsm/kg) perfused at 2 mL/min containing 150 mM NaCl, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 10 mM glucose, 3 mM potassium chloride, 2 mM calcium chloride, and 1.3 mM magnesium chloride

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[DOI] [PMC free article] [PubMed] [Google Scholar] 64.Senft AD, Bikoff EK, Robertson EJ, and Costello I (2019)

Medical conditions contributing to a deficiency include intestinal problems, gastric surgeries, atrophic gastritis, anemia, Crohns disease, celiac disease, lupus, and Graves disease

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