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10.1016/j.yebeh.2012.05.010 184 PeruccaE.HedgesA.MakkiK.RuprahM.WilsonJ.RichensA

While 97% of all proteins contain cysteine, reportedly only 18% of all proteins are expected to harbor disulfide bonds 19 other thiols are then free or otherwise modified

In general, shorter affinity tags (peptides) are more attractive as they are less likely to interfere with the expression, structure and function of the target protein, and their removal can be exempt, decreasing thus the overall costs of the purification process (Hearn and Acosta, 2001, Hedhammar et al., 2005, Terpe, 2003)

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