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Differentiation of the cells to myocytes was induced by incubating the cells in a standard differentiation medium consisting of DMEM with 2% horse serum (Thermo Fisher Scientific) for 6 days, and was confirmed by upregulation of mRNA expression of Myod1 and Myog ( 2.4 Assessment of mitochondrial function and cell viability Mitochondrial function was assessed by measuring adenosine triphosphate (ATP) content and mitochondrial membrane potential using ATP Assay Kit-Luminescence and JC-1 MitoMP Detection Kit, respectively, following the manufacturers instructions (Dojindo Laboratories, Mashiki, Japan)

This diffusion-driven mechanism suggestes that electron transfer is regulated by stochastic molecular collisions

Campbell-Walsh Urology , 9th edn., Vol 1

doi:10.1002/ptr.7729 Mozos I, Flangea C, Vlad DC, et al

References Fan, Y

Within the central nervous system (CNS), vitamin D signaling plays a fundamental role in brain development, neuroprotection, neurotransmission, and immunomodulation