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coli , the altered cysE gene encoding the Cys feedback inhibition-insensitive mutant SAT (T167A) 30 , the wild-type ydeD gene encoding inner membrane Cys exporter, and the altered serA gene encoding the l-serine feedback inhibition-insensitive mutant of d-3-phosphoglycerate dehydrogenase (T410stop)

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Notably, at concentrations of 25 nM or lower, cancer cell lines with high FR expression (HeLa) exhibited minimal detectable reduction, whereas those with lower FR levels (PC9) showed more pronounced depletion, suggesting that FolTAC activity may preferentially deplete FR in contexts where its expression is limited