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Genomic DNA processing, library preparation and sequencing Cell pellets from the screen were processed using the NucleoSpin Blood XL Kit (Macherey Nagel, 740950) according to manufacturers directions and extracted DNA was quantified with DeNovix Nanodrop

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Saccon RA, Bunton-Stasyshyn RK, Fisher EM, Fratta P

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We verified translocation of roGFP2 into the periplasm using fluorescence microscopy (Fig