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Vitamin C is a reducing agent capable of rapidly eliminating reactive oxygen species (ROS), it also works as a pro-oxidant antibacterial molecule, potentially altering the antimicrobial efficacy of some antibiotics by two proposed mechanisms: I- the transfer of vitamin C into bacterial cells, which results in the synthesis of H2O2 and, as a result, ROS

Two hours before completion of incubation 10 l of MTT (5 mg/ml) added to each well and allowed for formazan crystal formation

We demonstrated that the accumulated fumarate leads to oxidative stress by covalently binding to reduced GSH in a non-enzymatic reaction that forms succinicGSH

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In addition, molecular analyses identified CYSLTR1 and PTH1R as probable molecular targets of PP-NPs, suggesting receptor-mediated pathways as key contributors to the observed neurotoxicity [94]