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[DOI] [PubMed] [Google Scholar] 157.Clementi E., Brown G.C., Feelisch M., Moncada S

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To obtain the Retromer trimer, the GST-tagged Vps29 and Vps35 co-expressed cell pellet was mixed with His-Vps26A and resuspended in lysis buffer containing 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 2 mM 2-Mercaptoethanol, 50 g/ml benzamidine and DNase I before being processed through a Constant System TS-Series cell disruptor

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