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Tumor-intrinsic activity of chromobox 2 remodels the tumor microenvironment in high-grade serous carcinoma

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Then, the gel pieces were removed by centrifugation, and the protein-containing supernatants were collected and washed 3 times by Amicon Ultra-0.5 Centrifugal Filter Unit (3 kDa MWCO), to remove salts and impurities diffusing from the gel during the extraction