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After perfusion, tissue was either snap-frozen in liquid nitrogen, placed in 4% PFA for diffusion-fixation, or RNAlater (AM7021, Qiagen) for mRNA analysis

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We do not have any BPA (bisphenol A an industrial chemical that has been used to make certain plastics and resins) in our products [1] Sinha R, Sinha I, Calcagnotto A, et al

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Ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS, Waters Corp., Milford, United States) was used for sample analysis