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These compounds selectively blocked t-LTD at L4-L2/3 synapses with no effect on t-LTP at the same synapses, which was instead blocked by the GluN2A subunit-preferring antagonist NVP-AAM077

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1d) without altering ER levels (Extended Data Fig

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Antibody-bound complexes were then captured by incubation with protein A Sepharose CL-4B beads (Amersham, 17-0963-03) for rabbit antibodies or protein G Sepharose 4B beads (Invitrogen, 101241) for mouse antibodies (both 20 l per 1 ml of lysate) for 4 h at 4 C