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PSA % Free is the percentage of PSA not bound to proteins

To determine the pI of alanine, we take the average of these two pKa values: pI = (pKa of carboxyl group + pKa of amino group) / 2 pI = (2.35 + 9.69) / 2 6.02 Therefore, the isoelectric point of alanine is approximately 6.02

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