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They may look for signs of nervous system problems

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Published rodent in vivo studies have used intraperitoneal injection and oral (gavage) administration

Pooling across the field, a 2025 meta-analysis of seven randomized controlled trials, covering 456 participants, reported a standardized mean difference of -0.72 for wrinkle reduction, though the analysis noted high heterogeneity between studies and that roughly 40% of trials had industry funding both factors that warrant caution in interpreting the pooled effect size

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