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Holotranscobalamin (holoTC) concentrations, however, remain unchanged during pregnancy so the decline is unlikely to reflect B12 depletion

Notably, we observed an increase in the abundance of Aspergillaceae and a decrease in the abundance of Saccharomycopsis , Cystobasidium and Chaetomium (Figures 5C,D)

For Co-IP in protoplasts, proteins were expressed overnight in protoplasts by co-transfecting the indicated plasmids

(197) that could effectively cross the BBB and deliver medicine to the CNS

To assess the GSSG fraction, first, thiol groups of GSH are blocked with N-ethylmaleimide (NEM) in a subsample, followed by removal of excess NEM with toluene prior to reduction of GSSG to GSH with DTT and derivatization via mbrB (Kranner 1998