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doi: 10.1080/07391102.2020.1796811

A-D: Plasma total cysteine (tCys), reduced cysteine (rCys), cystine, and rCys/tCys ratio

Luciferin generation reagent (50 l/well) was added

Plus, while our bodies can produce some antioxidant compounds, production of those antioxidants naturally declines with age

Cointervention All pregnant women will also be given folic acid (0.4 mg) for the first 2 months of pregnancy followed by iron (60 mg elemental iron) and calcium supplements (500 mg) until 45 days after delivery according to the WHO guidelines.30 Recruitment procedure, confirmation of pregnancy and assessment of gestational age Prior and throughout the study period, we will identify pregnant mothers through a hospital and community-based surveillance system (figure 1)

doi: 10.3390/diagnostics12122979 Summary Keywords male infertility, ROS, oxidative stress, semen quality, steroidogenesis Citation Hussain T, Kandeel M, Metwally E, Murtaza G, Kalhoro DH, Yin Y, Tan B, Chughtai MI, Yaseen A, Afzal A and Kalhoro MS (2023) Unraveling the harmful effect of oxidative stress on male fertility: A mechanistic insight