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The supernatant was injected into a C18 LiChroCart column (5 mm x 250 mm x 4 mm) and the mobile phase applied at a flow rate of 1 mL min 1 , consisting of 50 mM sodium phosphate (pH 2.8), 10 mM heptane sulfonate and 10% acetonitrile

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J Am Acad Audiol