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Computational methods All DFT calculations were performed with the ORCA quantum chemistry package version 5.0 using the B3LYP functional, the def2-TZVP basis set for all atoms, and the CPCM solvation model 99,100,101,102

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Key metabolites identified in the dilated cardiomyopathy subtype

MASLD, Metabolically Associated Steatotic Liver Disease

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The pathway is activated by a three-order enzymatic cascade from MAP3K, MAPKK to MAPK, which regulates cell growth, differentiation, stress adaptation, and inflammation