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While the pathogenesis and mechanism underlying these corneal changes in TAO patients are likely multifactorial, the researchers proposed a few possible explanations

After slices were dehydrated in progressively stronger alcohols, cleaned in xylene, and mounted, hematoxylin was used as a counterstain ( Measurement of the percentage area of a positive SIRT -1 immunoreaction in hepatic tissue Images were taken using a digital camera (Toucan type BX53, Japan) linked to a computer and a light microscope (Olympus model BX53, Tokyo, Japan)

Ippolito et al., 2002

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