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While a modest shift in PD-L1 staining was observed in Myc-CaP cells following IGF-1 treatment, the overall MFI remained low compared to DU145 cells

Typhoon FLA 9500 biomolecular imager (GE Healthcare) was used to detect fluorescence of these gels as described below

Cell plates were washed twice to remove floating cells and treated with 500 g/ml Mitomycin C (M4287, Sigma Aldrich) to block proliferation
Psychiatry 10, 892

Fundamental & Clinical Pharmacology

[PubMed] [Google Scholar] 4.Bentley E, Mackie IC
