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The supernatant was then analyzed by HPLC with a Rainin Econosphere ODS C18 column and an acetonitrile/water mobile phase containing 0.1% trifluoroacetic acid

The Lancet, 395 (10218), 142155

Next, 3,3,5,5 Tetramethylbenzidine (TMB) substrate is used to enable visualization of the reaction and colour develops in proportion to the amount of rat p-IGF1R

Rosaneli CF, Bighetti AE, Antonio MA, Carvalho JE, Sgarbieri VC

Oncotarget 8(41):69493 Tsai CC, Wu SB, Kau HC, Wei YH (2018) Essential role of connective tissue growth factor (CTGF) in transforming growth factor-1 (TGF-1)-induced myofibroblast transdifferentiation from Graves orbital fibroblasts

Trigonelline is an NAD + precursor that improves muscle function during ageing and is reduced in human sarcopenia
