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doi: 10.1210/edrv.19.1.0324
This option works well when rib injury location makes local injection impractical
Injections help prevent or reverse this

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Return vial of HCG into the refrigerator until next use.

Fish were then incubated for 4 h in 1:500 Alexa Fluor 568 secondary antibody (Life Technologies) diluted in PBST at room temperature
