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Funding This work was supported by the National Natural Science Foundation (grant no

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The separate counting of small colonies (slowly growing, indicative of chromosome mutations) and large colonies (fast growing, indicative of gene mutations) also showed no significant increase in the number of colonies (absolute numbers) upon treatment with MA or EA (6487 small colonies upon treatment with 535 g MA, 7284 upon treatment with the same doses of EA, neither one of them dose-related, compared with 83 and 75 in the vehicle controls, respectively

Uraz S, Tahan V, Aygun C, Eren F, Unluguzel G, Yuksel M, Senturk O, Avsar E, Haklar G, Celikel C, Hulagu S, Tozun N (2008) Role of ursodeoxycholic acid in prevention of methotrexate-induced liver toxicity