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doi: 10.1172/JCI25790

WT C3H/HeN and Gsr / mice were kept at 25C in a specific pathogen-free vivarium with a 12-h alternating light-dark cycle
CYP450-mediated Metabolism of Mitragynine and Investigation of Metabolites in Human Urine

The entire procedure was carried out on ice to preserve protein activity and stability

[DOI] [PubMed] [Google Scholar] Sklan N.M., Barnsley E.A

Its action mitigates potential damage from byproducts like hydroxyl radicals and peroxynitrite, which can accrue under oxidative stress [34]
