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The cells were transfected with empty plasmid vectors or plasmids using Lipofectamine 2000 (Invitrogen, Waltham, MA, USA) according to the manufacturers instructions
Emerging evidence indicates a mechanistic interplay between the Nrf2Keap1 axis and the NLRP3 inflammasome, wherein Nrf2 activation not only counteracts oxidative stress but also suppresses NLRP3-mediated inflammatory signaling

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