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A cluster was considered enriched if the R o/e ratio was greater than 1

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The first step in the reaction is catalyzed by GCL, a heterodimer made up of a catalytic subunit (GCLC) that possesses the enzymes active site and performs the actual amino acid linkage, and a modulating subunit (GCLM) that regulates the activity of GCLC, as reviewed in Figure 1

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Low doses of gliotoxin, with a concentration of 1 M, exhibited 63% inhibition of the LLVY-amc peptide-hydrolyzing activity mediated by the 20S proteasome, suggesting the high immunosuppressive efficiency of gliotoxin [38]