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Among them, CTLA-4 and PD1/PD-L1 are important immune checkpoints for ovarian cancer (Mittica et al., 2016
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doi: 10.1186/1471-2407-12-293 152 LiuYLiWGuoMLiCQiuC
Amylin is secreted from pancreas with insulin and amylin receptor activation controls blood glucose and body weight [8]
Correct insertion yields a novel NcoI site generated by ligation of the blunted ends, and so NcoI (NEB) linearization of the supercoiled vector was used to determine correct insertion (Figure 1b, lower panel)
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