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Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the gold standard for quantifying dihexa in plasma, with a lower limit of quantification typically around 15 ng/mL depending on sample preparation and ionization efficiency

Scientific justification for timing: If, in exceptional circumstances, you propose conducting SP studies after initial human trials (Phase I), you'll need a strong scientific justification to explain your rationale to regulatory bodies

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Proton Pump Inhibitors, H2-Receptor Antagonists, Metformin, and Vitamin B-12 Deficiency: Clinical Implications

Also in this setting, the potency of FOXO4-DRI was more pronounced when applied in consecutive rounds (Fig