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First, because 4MP causes considerable phospho-JNK inhibition which negatively impacts p53 activation and necrosis 92 , we considered that 4MP causes a conservative 80% decrease of p53 activation and simulated this by multiplying the phosphorylation rate of p53 with parameter k 4MP JNK = 0.2 (i.e., decreasing the phosphorylation rate)

Modulation of Tyrosinase Expression Tyrosinase gene expression, together with Tyrp1 and Typr2, is induced by microphthalmia transcription factor (MITF), which is in turn regulated by signaling pathways, including cAMP/PKA/CREB, RAS/MEK/ERK, and -catenin pathways

[DOI] [PMC free article] [PubMed] [Google Scholar] 101.Barreto da Silva L., Camargo S.B., Moraes R.D.A., Medeiros C.F., Jesus A.M., Evangelista A., Villarreal C.F., Quintans-Jnior L.J., Silva D.F

J Gastrointest Cancer

Rapid deproteinization using acidic solutions (MPA, PCA, or trichloroacetic acid) immediately upon sample collection prevents enzymatic degradation and spontaneous oxidation