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Impaired acidification, fusion defects and reduced endosomeautophagosomelysosome pathway flux compromise the clearance of pathogenic aggregates, and raising cellular cAMP or free zinc has been proposed as a strategy to restore lysosomal pH and proteolytic capacity in affected neurons 166

doi: 10.1007/s10637-015-0309-4 189 ZengHXuQWangJXuXLuoJZhangLet al

Add solvent slowly along the inner wall of the vial to minimize foaming or peptide denaturation