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participate in the regulation of cytokines such as leukotrienes, macrophage metastasis inhibitory factor (MIF) and IL-2

The membranes were then incubated with horseradish peroxidase-linked secondary antibodies (Cell Signaling Technology) and the signals were visualized by enhanced chemiluminescence using an Amersham Imager 600 (GE Healthcare)

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It appears that dots were internalized and induced ROS, leading to apoptosis and necrosis (Shih et al., 2021)