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The excitation filter for CFP was 430/20 nm and emission filters for CFP and YFP were 485/20 nm and 535 nm/25 nm, respectively in microplate reader

Due to pandemic related safety concerns, the patients remained in quarantine within their home with all trial contact via phone, email, and web (REDCap), with contactless delivery of study packets via courier/mail

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The mitochondrial pellets were washed in 500 l of sucrose buffer

Cell viability assay for PDX-derived 3D cell cultures Ex vivo evaluation of cell viability in response to IACS-010759 exposure was performed in HBCx-137 derived cell cultures, following a protocol detailed elsewhere 62