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Cells were suspended in Live Cell Imaging Solution (LCIS, Thermo Fisher, Waltham, MA) containing 20 mM HEPES pH 7.4, 140 mM NaCl, 2.5 mM KCl, 1.8 mM CaCl 2 , 1 mM MgCl 2 , supplemented with high glucose (4.5 g/l)

Sadly for us, natural production declines as we hit our thirties which is why many resort to supplementation

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10.1016/j.jprot.2018.04.012 27 ClarkS

Genomics 20, 513516

Brain injury in different regions was evaluated using a semiquantitative neuropathological scoring system as described [52] (Table 1), providing a common score 08, grade 0 indicates no histopathologic damage