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Subsequently, cells were washed in Tris Buffered Saline (TBS) and permeabilized for 25 min in 2% Triton X-100 in TBS, depending on the antibody
Statistical analyses GraphPad Prism 8 was used for all statistical analyses
The IV method bypasses the digestive system, offering direct absorption and quicker results
Micronuclear envelopes are prone to rupture due to the lack of a stable nuclear lamina, releasing dsDNA into the cytoplasm [44]
A glutathione IV drip will usually take around 30-60 minutes, though cocktails with more and more varied ingredients can take longer
These results indicated that Sirt1 regulated the nuclear translocation of Gli-1 and the expression levels of components of the Shh signaling pathway in N9 microglia following OGD/R injury in vitro
