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10.1038/s41586-021-03539-7 87 MurataH.TakamatsuH.LiuS.KataokaK.HuhN

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Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles

Nat Rev Endocrinol (2023) 19, 722740

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Ehrlich AK, Pennington JM, Bisson WH, Kolluri SK, Kerkvliet NI
