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Worm pellets were resuspended in 150 L cold extraction buffer (16 mM KH 2 PO 4 , 84 mM K 2 HPO 4 , 8.8 mM EDTA, 2 mM NEM, 1% Triton X-100, 0.6% SSA) and samples were subjected to three freezethaw cycles (1 min liquid nitrogen, 1 min 37 C water bath) followed by homogenizing four times for 20 s using a Bead Ruptor

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56 Expression of cell surface adhesion molecules is very important for the migration of neuronal cells in the SVZ-rostral migratory stream system

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The balance between normal cellular oxidation and antioxidants must be maintained, as excess copper levels can induce oxidative stress, leading to cell death and cardiovascular disease (14)

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