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The mean change of 387.7 pg/mL was significant ( p = 0.0001 in Students t -test)

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Particles can come from: Degraded peptide forming insoluble aggregates Contamination during manufacturing or storage Rubber stopper fragments from repeated needle punctures Crystallization of dissolved components If you see particles, do not try to filter them out

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doi: 10.1016/j.actbio.2014.06.015