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Previous studies indicate that airway epithelial cells can respond to melatonin via MT1 to reduce alarmin production (18), whereas airway smooth muscle hyperreactivity may be enhanced through MT2 signaling (60)

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The indicated proteins were incubated in pull-down buffer (20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA and 0.2% Triton X-100) for 1 h with gentle shaking at 4 C

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The aim is to establish a balance between the damage caused by chronic inflammation and the protective effects of the host inflammatory defense system